XI. OLE Beverage Toxicological Experiment Report & XII. The Slide Records Of Mice Sperm Abnormality Test

1. MATERIALS AND PROCEDURES

1.1 Sample:
The OLE beverages were provided by American Health Product, INC.

1.2 Experimental animals:
Healthy male Kunming mice obtained from the animal breeding unit of Cancer Institute and Hospital of Chinese Academy of Medical Science and PekingUnionMedicalCollege were used for the study.

1.3. Mice Sperm Abnormality Test:
50 sexually matured male mice with 30-50g body weight were selected and randomly assigned to 5 groups. The positive controls were fed with 40mg/kg.b.w. of Cyclophosphamide, negative controls were fed with distilled water; OLE beverages with feeding dose of 1.25 g/kg.b.w., 2.50 g/kg.b.w., and 5.00g/kg.b.w. were given and distilled water was added to prepare the desired concentration. The fed was administered by gavage once a day for 5 days consecutively; then the mice were killed after 30 days after the final feeding. The epididymis was collected to prepare slide with HE staining. 5 mice from each groups was randomly selected, 1000 sperms with complete structure from each selected mice were evaluated and the incidence of abnormal sperm was counted in percentage. Statistical analysis was done

2. RESULT

Mice Sperm Abnormality Test:
In the case of the incidence of sperm abnormality, the table below has shown that there was no significant difference between the OLE-treated group and negative controls (P>0.05), while there was significant difference between the cyclophosphamide-treated group and negative controls (P<0.01). Therefore, the result has shown no sperm abnormality in the OLE- treated groups.

Table 4: The Incidence of Sperm Abnormality in Selected Mice

Dosage

(g/kg.b.w)

Animal count

Sperm count

Abnormality sperm count

Incidence of sperm abnormality

Control

0.00

5

5000

86

1.72

Low

1.25

5

5000

74

1.48

Medium

2.50

5

5000

84

1.68

High

5.00

5

5000

76

1.52

40mg/kg.b.w cylophosphamide

5

5000

377

7.54

* There were significant differences when compared with the negative controls (P<0.01)

3.  SUMMARY

Mice Sperm Abnormality Test: The result was shown to be negative.

 

XII.  The Slide Records of Mice Sperm Abnormality Test

Dosage Group: High (5.00g/kg.b.w.)

       Abnormal

       Sperm

 

Sample No.

Hookless Sperm

AmorphousSperm Banana-shaped head Sperm Macro cephalic Sperm Double- headed Sperm Double- tailed Sperm Kinked-tailSperm

Total(%)

1-1

4

2

4

3

0

0

0

13

1-2

4

5

3

4

0

0

0

16

1-3

2

12

3

1

0

0

0

18

1-4

3

9

1

0

0

0

0

13

1-5

5

8

3

0

0

0

0

16

Total

18

36

14

8

0

0

0

76

Sperm Abnormality (%)

1.8

3.6

1.4

0.8

0

0

0

1.52

 

Dosage Group: Medium (2.50g/kg.b.w.)

       Abnormal

       Sperm

 

Sample No.

Hookless Sperm AmorphousSperm Banana-shaped head Sperm Macro cephalic Sperm Double -headed Sperm Double -tailed Sperm Kinked -tailSperm Total(%)
2-1

1

7

3

1

0

0

0

12

2-2

3

6

4

5

0

0

0

18

2-3

3

11

3

0

0

0

0

17

2-4

5

8

0

0

0

0

0

13

2-5

0

14

10

0

0

0

0

24

Total

12

46

20

6

0

0

0

84

Sperm Abnormality (%)

1.2

4.6

2

0.6

0

0

0

1.68

 

Dosage Group: Low (1.25g/kg.b.w.)

       Abnormal

       Sperm

 

Sample No.

Hookless Sperm

AmorphousSperm Banana-shaped head Sperm Macro cephalic Sperm Double -headed Sperm Double -tailed Sperm Kinked -tailSperm

Total(%)

3-1

1

7

4

0

0

0

0

12

3-2

1

9

3

1

0

0

0

14

3-3

1

8

7

1

0

0

0

17

3-4

1

10

5

2

0

0

0

19

3-5

1

8

2

1

0

0

0

12

Total

5

42

21

5

0

0

0

74

Sperm Abnormality (%)

0.5

4.2

2.1

0.5

0

0

0

1.48

 

Dosage Group: Negative Controls

       Abnormal

       Sperm

 

Sample No.

Hookless Sperm AmorphousSperm Banana-shaped head Sperm Macro cephalic Sperm Double -headed Sperm Double -tailed Sperm Kinked -tailSperm Total(%)
4-1

4

7

2

0

0

0

0

13

4-2

5

14

1

0

0

0

0

20

4-3

5

5

10

1

0

0

0

21

4-4

2

19

1

1

0

0

0

23

4-5

3

4

2

0

0

0

0

9

Total

19

49

16

2

0

0

0

86

Sperm Abnormality (%)

1.9

4.9

1.6

0.2

0

0

0

1.72

 

Dosage Group: Cyclophosphamide-treated Group (Positive Controls)

       Abnormal

       Sperm

 

Sample No.

Hookless Sperm AmorphousSperm Banana-shaped head Sperm Macro cephalic Sperm Double -headed Sperm Double -tailed Sperm Kinked -tailSperm Total(%)
5-1 2 62 3 9 0 0 0 76
5-2 5 61 4 6 1 0 1 78
5-3 12 61 4 5 1 0 0 83
5-4 15 40 4 6 0 0 0 65
5-5 5 61 1 7 0 0 1 75
Total 39 285 16 33 2 0 2 377
Sperm Abnormality (%) 3.9 28.5 1.6 3.3 0.2 0 0.2 7.54

 

The Incidence of Sperm Abnormality in Selected Mice

Dosage

(g/kg.b.w)

Animal Count

Sperm Count

Abnormality Sperm Count

Incidence of Sperm Abnormality (%)

Positive Control

5

5000

86

1.72

Low

5

5000

74

1.48

Medium

5

5000

84

1.68

High

5

5000

76

1.52

40mg/kg.b.w cylophosphamide

5

5000

377

7.54

Health Supplement Anti  Aging Supplement Antioxidant Supplement What is OLE

 Home    Antioxidant   Biocell Collagen II    Health Screening     Blog     Contact Us     Franchise